Effect of RNAi-induced inhibition of ets-1 gene expression on invasion properties of oral squamous carcinoma cells-Functional evaluation of ets-1 gene-

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  • AKANE Mitsunori
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • OHNISHI Yuichi
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • KAKUDO Kenji
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University

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Other Title
  • 口腔扁平上皮癌細胞の浸潤能に及ぼすＲＮＡｉを用いたｅｔｓ‐１遺伝子発現抑制の影響―ｅｔｓ‐１遺伝子の機能評価―
  • コウクウ ヘンペイ ジョウヒガン サイボウ ノ シンジュンノウ ニ オヨボス RNAi オ モチイタ ets 1 イデンシ ハツゲン ヨクセイ ノ エイキョウ ets 1 イデンシ ノ キノウ ヒョウカ
  • Functional evaluation of<I>ets</I>-1 gene
  • <I>ets</I>-1遺伝子の機能評価

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Abstract

Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes, such as matrix metalloproteinases (MMPs). It is known thatetsproto-oncogenes exist in transcriptional regulation refions of the MMP gene and enhance or inhibit MMP transcription. In this study, we restrained theets-1 gene expression of an oral squamous carcinoma cell line by means of RNA interference (RNAi) and examined the change in invasion ability from a molecular biological perspective. An oral squamous carcinoma cell line (HSC-3) was transfected with short interfering RNA (siRNA) ofets-1. The invasive ability of the transfected cells was determined by a matrigel invasion assay, and MMP-9 production was measured by gelatin zymography. These data were compared with those of normal cultured cells. Matrigel invasion assay demonstrated that the transfected cells had a lower invasive ability than HSC-3, and gelatin zymography demonstrated that the MMP-9 production in these cells had decreased. These results suggested that transfection of siRNAets-1 would inhibit tumor invasion through down-regulation of MMP-9.

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