FNA-FISH法による口腔扁平上皮癌におけるEGFR遺伝子の数的異常の解析 Analysis of epidermal growth factor receptor (EGFR) gene numerical aberration in oral squamous cell carcinomas by FNA-FISH technique
Overexpression and amplification of the epidermal growth factor receptor (EGFR) frequently occur in many malignant tumors, including oral squamous cell carcinomas (SCCs). Several studies have reported that abnormalities of this gene may be a valuable biologic marker of a poor prognosis. To investigate numerical aberration of the EGFR gene, we performed fluorescence in situ hybridization (FISH) with specimens obtained by fineneedle aspiration (FNA) biopsies of 57 primary oral SCCs. The EGFR numerical aberrations were identified in 24 (42.1 %) of the 57 patients with primary oral SCCs. Twenty-one (36.8 %) were polysomic for the EGFR gene, and amplification of this gene was found in 3 (5.3 %) of the tumors. The EGFR numerical aberrations were associated significantly with histopathologic grade (p=0.001), the mode of invasion (p=0.001), disease recurrence (p=0.001), and survival (p=0.002). Moreover, disease-free survival rate and overall survival rate in the patients with EGFR numerical aberrations were significantly lower than those in the patients without EGFR numerical aberrations (p=0.0020 and p=0.0021). Therefore, analysis of numerical aberrations of the EGFR gene by FISH with tissues derived from fine-needle biopsy may be a rapid and useful method for predicting poor outcomes in patients with oral SCCs.
日本口腔外科学会雑誌 52(12), 684-688, 2006-12-20
Japanese Society of Oral and Maxillofacial Surgeons