多血小板血漿の血小板凝集能とTGF-β含有量に関する解析 Analysis of platelet aggregation and TGF-β content in platelet-rich plasma
Recently, platelet-rich plasma (PRP) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of PRP from small quantities of blood.<BR>Various conditions were used for anticoagulants and centrifugal separation speed for blood samples obtained from 23 healthy subjects to investigate optimal concentration conditions. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r. p. m. and 1300 r.p.m. The effectiveness of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after double centrifugation.
日本口腔外科学会雑誌 52(6), 316-321, 2006-06-20
Japanese Society of Oral and Maxillofacial Surgeons