GFP遺伝子導入ラット舌扁平上皮癌細胞株を用いた肺微小転移に対するTS-1の転移抑制効果 Effects of TS-1 on lung micrometastases as evaluated with a green fluorescent protein (GFP)-tagged rat tongue squamous carcinoma cell line

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著者

    • 関 泰 SEKI Yasushi
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 中西 速夫 NAKANISHI Hayao
    • 愛知県がんセンター研究所腫瘍病理学部 Division of Oncological Pathology, Aichi Cancer Center Research Institute
    • 阿部 厚 [他] ABE Atsushi
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 大野 ふみ OHNO Fumi
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 木下 篤敬 KINOSHITA Atsutaka
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 竹内 伸一 TAKEUCHI Shinichi
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 田中 あづさ TANAKA Adusa
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 安井 昭夫 YASUI Akio
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University
    • 立松 正衞 TATEMATSU Masae
    • 愛知県がんセンター研究所腫瘍病理学部 Division of Oncological Pathology, Aichi Cancer Center Research Institute
    • 栗田 賢一 KURITA Kenichi
    • 愛知学院大学歯学部口腔外科学第1講座 The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University

抄録

[Purpose] Lung metastases in patients with tongue cancer are resistant to various therapies, resulting in poor outcomes. In the present study, we examined whether such lung metastases are controllable by adjuvant chemotherapy targeting micrometastases in the lung. We used a micrometastasis model using a rat tongue carcinoma cell line (RSC3) tagged with the green fluorescent protein gene (RSC3LM-GFP) in nude mice.[Methods] Lung metastases were formed after intravenous (i. v.) injection of 1 (3)×10<SUP>5</SUP> tumor cells in a low (high) metastasis model. TS-1 (5-fluorouracil derivative)(20mg/kg) was orally administered from day 1 Postinjection (early administration) and from day 7 post-injection (late administration) 5 times per week for 3 weeks. Mice bearing lung metastases underwent autopsy 4 weeks after injection, and the lungs were removed. The numbers of lung metastases were determined by GFP fluorescence.[Results] Lung metastasis including micrometastasis could be clearly visualized under illumination with blue light. In the low metastasis model, GFP-positive metastatic nodules in the lung were more strongly reduced by early TS-1 oral administration than by late administration (p<0.003). Survival was also much longer in mice with early TS-1 treatment than in those with late treatment in the low metastasis model (p<0.02), but not in the high metastasis model.[Conclusion] These results indicate that the sensitivity of lung metastases of tongue carcinoma to TS-1 depends on the timing of drug administration and the number of metastases. Small numbers of micrometastases in the lung at an early stage are most susceptible and can be effectively eliminated by chemotherapy targeting micrometastasis, leading to longer survival of mice.

収録刊行物

  • 日本口腔外科学会雑誌  

    日本口腔外科学会雑誌 52(4), 216-224, 2006-04-20 

    Japanese Society of Oral and Maxillofacial Surgeons

参考文献:  22件

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各種コード

  • NII論文ID(NAID)
    10018860025
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • NDL 記事登録ID
    7941619
  • NDL 雑誌分類
    ZS44(科学技術--医学--歯科学・口腔外科学)
  • NDL 請求記号
    Z19-145
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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