Automatic Mapping of Viable Microbial Cells Distributed in the Surface Layer of Cotton Fabrics

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著者

    • KOZONE IKUKO
    • Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
    • SAITO MIKAKO
    • Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
    • MATSUOKA HIDEAKI
    • Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

抄録

Viable microbial cells distributed in a 130 μm thick surface layer of cotton fabrics were stained with a fluorescent glucose, 2- [<I>N</I>-(7-nitrobenz-2-oxa-1, 3-diazol-4-y1) amino] -2-deoxy-Dglucose (2-NBDG), and automatically mapped with an ultra-deep focusing range microscope (UDF) system. The software of the UDF system was upgraded and the number of <I>Candida albicans</I> cells could be counted at a higher precision than before. Bacterial cells of <I>Pseudomonas fluorescens</I>, <I>Serratia marcescens</I>, and <I>Citrobacter freundll</I>, which were smaller than 1-2μm, were successfully mapped for the first time. These results indicate the practical importance of the present method in the evaluation of the antibacterial properties of fabrics and the efficacy of washing.

収録刊行物

  • Biocontrol science  

    Biocontrol science 12(1), 31-34, 2007-03-01 

    The Society for Antibacterial and Antifungal Agents, Japan

参考文献:  14件

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各種コード

  • NII論文ID(NAID)
    10018870857
  • NII書誌ID(NCID)
    AA11169621
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424815
  • データ提供元
    CJP書誌  J-STAGE 
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