Automatic Mapping of Viable Microbial Cells Distributed in the Surface Layer of Cotton Fabrics

• FUJIOKA KOHTARO

  Kobe Technical Center, Procter & Gamble Far East, Inc. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• KOZONE IKUKO

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• SAITO MIKAKO

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• MATSUOKA HIDEAKI

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

抄録

Viable microbial cells distributed in a 130 μm thick surface layer of cotton fabrics were stained with a fluorescent glucose, 2- [N-(7-nitrobenz-2-oxa-1, 3-diazol-4-y1) amino] -2-deoxy-Dglucose (2-NBDG), and automatically mapped with an ultra-deep focusing range microscope (UDF) system. The software of the UDF system was upgraded and the number of Candida albicans cells could be counted at a higher precision than before. Bacterial cells of Pseudomonas fluorescens, Serratia marcescens, and Citrobacter freundll, which were smaller than 1-2μm, were successfully mapped for the first time. These results indicate the practical importance of the present method in the evaluation of the antibacterial properties of fabrics and the efficacy of washing.

収録刊行物

• Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ

  Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ 12 (1), 31-34, 2007

  日本防菌防黴学会

キーワード

• Ultra-deep focusing range (UDF) fluorescent microscope
• A fluorescent glucose
• Viable cell imaging
• Cell deposition on fabrics

詳細情報

• CRID: 1390282679440980992
• NII論文ID: 10018870857
• NII書誌ID: AA11169621
• DOI: 10.4265/bio.12.31
• ISSN: 18840205
• ISSN: 13424815
• PubMed: 17408006
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可