Immunolocalization of Inhibin/Activin Subunits and Steroidogenic Enzymes in Equine Neonatal Ovaries

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  • NAKAI Rie
    Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • WENG Qiang
    Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University Faculty of Biological Science and Technology, Beijing Forestry University
  • TSUNODA Nobuo
    Shadai Corporation
  • TANIYAMA Hiroyuki
    Department of Veterinary Pathology, Faculty of Veterinary Medicine, Rakuno Gakuen University
  • WATANABE Gen
    Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • TAYA Kazuyoshi
    Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology

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The objective of this study was to investigate the cellular immunolocalization of inhibin α and inhibin/activin (βA and βB) subunits and steroidogenic enzymes in neonatal equine ovaries. The ovaries were obtained from two equine neonates of 3 days and 18 days of age. The sections of ovarian tissues were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin α, inhibin/activin βA and inhibin/activin βB and bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3β-hydroxysteroid dehydrogenase (3βHSD), porcine testicular 17α-hydroxylase cytochrome P450 (P450c17), and human placental aromatase cytochrome P450 (P450arom). Inhibin α and inhibin/activin βA subunits, but not inhibin/activin βB subunits were expressed in the ovarian interstitial cells of equine neonates. P450scc and P450c17 were also identified in the ovarian interstitial cells and P450arom with weak immunostaining. However, 3βHSD was not found in the ovarian interstitial cells of the equine neonates. These results suggest that equine neonatal ovaries have the ability to synthesize inhibin A and steroid hormones, which may play some important physiological roles in the developing ovaries of equine neonates.<br>

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