タンパク質分析のための最新質量分析装置　　タンデム四重極リニアイオントラップを用いた微量タンパク質の検出及び翻訳後修飾研究への戦略４０００Ｑ　ＴＲＡＰシステム

• 土屋 文彦

  アプライドバイオシステムズジャパン株式会社

書誌事項

タイトル別名

• Novel strategy for PTM study and detection of low abundant protein using tandem quadrupole linear ion trap mass spectrometer.
• タンデム四重極リニアイオントラップを用いた微量タンパク質の検出及び翻訳後修飾研究への戦略4000 Q TRAPシステム
• タンデム 4ジュウキョク リニア イオン トラップ オ モチイタ ビリョウ タンパクシツ ノ ケンシュツ オヨビ ホンヤクゴ シュウショク ケンキュウ エノ センリャク 4000 Q TRAP システム

抄録

Mass spectrometry has become the main technique in proteomics study. And the strategy has been recently changed to focus more on research of target proteins from comprehensive proteins, because characterization of target proteins and quantitative study of protein biomarkers could provide important knowledge for drug discovery and development. Protein biomarkers and PTM (post-translational modification) are the main targets in target protein research called focused proteomics. However, biomarker discovery and PTM research are challenging due to difficulty in detection. A phosphate group of a compound could decrease ionization efficiency, which makes the detection of phosphorylated proteins more difficult. Most biomarkers are low abundant proteins, so detection of such compounds from highly complex samples like serum and cell lysate is not easy without sample fractionation using multi dimensional LC. The difficulty of proteomics technology using mass spectrometry results from lack of a tool to detect target proteins directly in complex samples. Here we introduce a strategy to detect a target protein directly even in a low concentration sample (e.g., PTM sample and low abundant biomarker) without additional fractionation. In this strategy unique scan modes of 4000 Q TRAP^® system, precursor ion scan and MRM are key features to remove contaminant signal in chromatogram. We also show successful examples of the detection of phosphorylated peptides from digested protein kinase, the verification of low abundant protein using a new tool, "MIDAS^TM workflow", and the monitoring for variation of phosphorylation sites using iTRAQ^TM reagent.<br>

収録刊行物

• 生物物理化学

  生物物理化学 51 (1), 23-35, 2007

  日本電気泳動学会

キーワード

• QTRAP
• PTM
• MRM
• phosphorylation
• quantification

詳細情報

• CRID: 1390282679180409600
• NII論文ID: 130004456444
• NII論文ID: 10018874528
• NII書誌ID: AN00129729
• DOI: 10.2198/sbk.51.23
• COI: 1:CAS:528:DC%2BD2sXktFGjsb4%3D
• ISSN: 13499785
• ISSN: 00319082
• NDL書誌ID: 8746098
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8746098
• Web Site: https://search.jamas.or.jp/link/ui/2007194501
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可