The Dugesia ryukyuensis Database as a Molecular Resource for Studying Switching of the Reproductive System

• Ishizuka, Hideyuki

• Maezawa, Takanobu

• Kawauchi, Junpei

• Nodono, Hanae

• Hirao, Yukako

• Nishimura, Osamu

• Nakagawa, Haruka

• Sekii, Kiyono

• Tasaka, Kenta

• Tarui, Hiroshi

• Agata, Kiyokazu

• Hoshi, Motonori

• Kobayashi, Kazuya

• Sakakibara, Yasubumi

• Matsumoto, Midori

Abstract

The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.

Journal

• Zoological Science

  Zoological Science 24 (1), 31-37, 2007-01

  Zoological Society of Japan

Keywords

• planarian
• EST analysis
• sexualization
• asexual-sexual switch
• gene ontology

Details

• CRID: 1050282677087584384
• NII Article ID: 10018877878
• NII Book ID: AA10545874
• ISSN: 02890003
• DOI: 10.2108/zsj.24.31
• HANDLE: 2433/85330
• NDL BIB ID: 8690281
• PubMed: 17409714
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8690281
• Web Site: http://www.bioone.org/doi/pdf/10.2108/zsj.24.31
• Text Lang: en
• Article Type: journal article
• Data Source:
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  • PubMed
  • CiNii Articles
  • KAKEN
  • Integbio