Determination of a complete lemur mitochondrial genome from feces

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抄録

Mitochondrial genome (mt-genome) sequences are widely used in molecular phylogenetic studies because of their relatively small size and of the strict orthology of their genes. It is sometimes the most difficult step in such studies to get samples particularly from endangered species. Here, we report successful amplification and sequencing of mt-genome of <i>Propithecus</i> (sifaka) from feces sample, using the extract method of Chelex-100 Phenol-Chloroform or QIAamp DNA Stool kit (Qiagen) in combination with FTA cards (Whatmann). For biologists, such a noninvasive sampling method should be an important resource that will provide greater opportunities to collect invaluable samples. By using the mt-genome sequence of sifaka obtained by this work with other published sequences of primates, we estimated the phylogeny of primates, and demonstrated that the evolutionary rate acceleration occurred in the Anthropoids lineage after they diverged from tarsier.<br>

収録刊行物

  • Mammal study = The Continuation of the Journal of the Mammalogical Society of Japan  

    Mammal study = The Continuation of the Journal of the Mammalogical Society of Japan 32(1), 7-16, 2007-06-01 

    日本哺乳類学会

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各種コード

  • NII論文ID(NAID)
    10018878846
  • NII書誌ID(NCID)
    AA11185060
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13434152
  • NDL 記事登録ID
    8690189
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z54-G149
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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