Lanthanide Complex Strategy for Detection and Separation of Histidine-tagged Proteins
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A series of trivalent lanthanide complexes were immobilized on SPR sensory chips and protein separation membranes. They worked as alternatives to transition-metal complexes in detection and separation of histidine-tagged ubiquitin protein. Since the highly coordinated complex between NTA ligand, Eu<SUP>3+</SUP> center, and hexahistidine moiety exhibited the enhanced luminescence, the present system provides a further possibility for use in in-situ optical protein imaging process.
- Chemistry Letters
Chemistry Letters 36(4), 554-555, 2007-04-05
The Chemical Society of Japan