Analysis of mutations of inflammatory cytokine and Toll-like receptor genes in periodontitis in Down syndrome patients

• Oomori Yuriko

  Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University

• Imamura Yasuhiro

  Department of Pharmacology, Matsumoto Dental University

• Fujigaki Yoshihisa

  Department of Community Dentistry, Matsumoto Dental University

• Hosaka Kazuo

  Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University

• Miyazawa Hiroo

  Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University

• Kasahara Hiroshi

  Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University

• Wang Pao-Li

  Department of Pharmacology, Matsumoto Dental University

Abstract

Periodontitis is a widespread and serious dental disease that results from multifactorial and lifestyle-related causes. Patients with Down syndrome have a high risk of periodontal disease from an early age, but little is known about the genetic factors that may cause or exacerbate it. With this regard, we isolated DNAs from lingual mucosal cells and examined the frequency of specific single nucleotide polymorphisms (SNPs) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The SNPs on which we focused were of the pro-inflammatory cytokine genes Interleukin (IL)-1A(-889) and IL-1B(+3953), and of the Toll-like receptor genes (TLR)2(Arg677Trp) and TLR4(Asp299Gly). The subjects of the study, all of Japanese extraction, were: (i) 18 individuals with Down syndrome and periodontitis; (ii) 10 individuals with Down syndrome and no periodontitis; (iii) 20 control individuals without Down syndrome but with periodontitis; and (iv) 10 control individuals without periodontitis. The patterns of occurrence of SNPs of the IL-1A, IL-1B, TLR2 and TLR4 were similar in all groups.

Journal

• Pediatric Dental Journal

  Pediatric Dental Journal 17 (1), 19-26, 2007

  The Japanese Society of Pediatric Dentistry

Keywords

• Down syndrome
• Interleukin-1
• Periodontitis
• Single nucleotide polymorphism
• Toll-like receptor

Details

• CRID: 1390282679360794624
• NII Article ID: 130004475812
• NII Article ID: 10018904361
• NII Book ID: AA10809637
• DOI: 10.11411/pdj.17.19
• ISSN: 18803997
• ISSN: 09172394
• Web Site: https://search.jamas.or.jp/link/ui/2008016320
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed