Observations of pulpotomy in rats using in vivo Micro-CT-The changes after treatment of formocresol and calcium hydroxide pulpotomies or CO2 laser irradiation-

• Yang Jing

  Department of Oral Health Promotion, Institute for Oral Science, Matsumoto Dental University

• Osuga Naoto

  Department of Oral Health Promotion, Institute for Oral Science, Matsumoto Dental University Department of Pediatric Dentistry, Matsumoto Dental University

• Li Xianqi

  Department of Hard Tissue Research, Institute for Oral Science, Matsumoto Dental University

• Wang Raorao

  Department of Pediatric Dentistry, Matsumoto Dental University

• Xu Qiang

  Department of Pediatric Dentistry, Matsumoto Dental University

• Yanagisawa Shigeru

  Department of Oral Health Promotion, Institute for Oral Science, Matsumoto Dental University

• Nakade Toshiyuki

  Department of Pediatric Dentistry, Matsumoto Dental University

• Kida Akio

  Department of Pediatric Dentistry, Matsumoto Dental University

• Arai Yoshinori

  Department of Hard Tissue Research, Institute for Oral Science, Matsumoto Dental University

• Miyazawa Hiroo

  Department of Oral Health Promotion, Institute for Oral Science, Matsumoto Dental University Department of Pediatric Dentistry, Matsumoto Dental University

書誌事項

タイトル別名

• —The changes after treatment of formocresol and calcium hydroxide pulpotomies or CO<SUB>2</SUB> laser irradiation—

抄録

In general, pulpotomy is evaluated by pathological examination of sacrificed animals at each observing stage. Not only lots of small animals have to devote itself to experimentation, but also the result of research is lacking about the serial observation of an individual experimental animal. The aim of this study was to compare the relative effectiveness of the calcium hydroxide, formocresol and CO₂ laser using the R_mCT^®. Twenty male Wistar rats, 8 weeks of age, were used and imaged in present study. They were serially observed using the R_mCT^® on days 0, 3, 10, 30, 50 after pulpotomy, followed pathohistological examination. The R_mCT^® images revealed that the pulp capping agents were partly absorbed and a hard tissue-like was observed immediately beneath it, especially in the CO₂ laser group. Pathohistological examination showed a hard tissue-like appearance and inflammatory cell infiltration. The R_mCT^® images agreed with pathohistological finding on the days 50 after pulpotomy. Our research suggested that the R_mCT^® has made it possible to scan serial imaging of small animals such as experimental rats under anesthesia. Since its higher resolution enables clear observation of the entire hand of the rat, it is possible not only to compare the effectiveness of each pulpotomy method, but also to serially observe a single experimental animal during the process post-pulpotomy healing, growth, and aging. This study answered well for the animal protective law that to use the least of animal to got the best result.

収録刊行物

• Pediatric Dental Journal

  Pediatric Dental Journal 17 (1), 32-39, 2007

  一般社団法人 日本小児歯科学会

キーワード

• Calcium hydroxide
• CO<SUB>2</SUB> laser
• Formocresol
• Longitudinal changes
• Pulpotomy
• R_mCT<SUP>®</SUP>

詳細情報

• CRID: 1390282679360797824
• NII論文ID: 130004475814
• NII論文ID: 10018904419
• NII書誌ID: AA10809637
• DOI: 10.11411/pdj.17.32
• ISSN: 18803997
• ISSN: 09172394
• Web Site: https://search.jamas.or.jp/link/ui/2008016322
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可