Cross-reactivity of Monoclonal Antibodies on Peripheral Blood Cells from Common Marmosets by Flow Cytometry.

DOI 5 References
  • Hayashi Sunao
    Pharmaceutical Development Laboratories, Development Department, Pharmaceutical Division, Kirin Brewery Co., Ltd.
  • Kikuchi Yasuko
    Pharmaceutical Development Laboratories, Development Department, Pharmaceutical Division, Kirin Brewery Co., Ltd.
  • Harada Katsuhiko
    Pharmaceutical Development Laboratories, Development Department, Pharmaceutical Division, Kirin Brewery Co., Ltd.
  • Kawahara Jun-ichi
    Pharmaceutical Development Laboratories, Development Department, Pharmaceutical Division, Kirin Brewery Co., Ltd.

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  • フローサイトメトリーを用いたコモンマーモセット末梢血における市販抗体の交差反応性の検討

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Abstract

<p>The Common marmoset becomes increasingly important in the development of recombinant monoclonal antibody drugs, which react with only primates, because of both their relatively close immunological relationship with humans and their relative small size. However, there is little information about cross-reactivity of commercial monoclonal antibodies with Common marmosets for lymphocyte subset analysis. In this study,we compared cross-reactivity with Common marmosets, Cynomolgus monkeys and humans using 19 commercial monoclonal antibodies. Furthermore, we optimized the method of preparation of Common marmoset peripheral blood in flow cytometric analysis. Eleven of 19 monoclonal antibodies that react with human or monkey antigens of CD-defined molecules were cross-reactive with Common marmoset antigens. Those antibodies that cross-reacted with Common marmosets were sufficient for lymphocyte subset analysis. Five lysing solutions were used for preparation of Common marmoset peripheral blood. By using Red Blood Cell Lysing Buffer, Common marmoset peripheral blood cells can be separated to each fraction of cells while maintaining cell viability. The minimum quantity of Common marmoset peripheral blood was only 25 to 50 μl for flow cytometric analysis of lymphocyte subsets. This flow cytometric method using Common marmoset peripheral blood can be used to speculate on the potential immunotoxic effect of monoclonal antibody drugs on humans.</p>

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