2種類のヒラメ免疫グロブリンの精製および Lactococcus garvieae の抗原解析 Purification of Two Different Immunoglobulins (Igs) from Olive Flounder Paralichthys olivaceus and Analysis of Lactococcus garvieae Antigens by the Igs

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Immunoglobulins (Igs) were purified from sera obtained from olive flounder <i>Paralichthys olivaceus</i> immunized with goat IgG using immunoaffinity and mannan-binding protein (MBP) affinity columns and designated IMMIg and MBPIg respectively. SDS-PAGE and two-dimensional gel electrophoresis (2-DE) analyses demonstrated that the olive flounder serum contained at least two different types of Ig in terms of molecular weight and pI of heavy chain. 2-DE separation of the Igs followed by peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) revealed that these two Igs were IgM and IgM precursor of olive flounder. Monoclonal antibodies (MAbs) were raised against the IMMIg and MBPIg and proteome analysis of Lactococcus garvieae using these MAbs and olive flounder immune sera demonstrated that IMMIg and MBPIg recognized different antigens of L. garvieae. This suggested that different Igs are possibly involved in protecting olive flounder against L. garvieae infection.

収録刊行物

  • 魚病研究  

    魚病研究 42(1), 19-28, 2007-03-15 

    The Japanese Society of Fish Pathology

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各種コード

  • NII論文ID(NAID)
    10018909268
  • NII書誌ID(NCID)
    AN00063165
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0388788X
  • NDL 記事登録ID
    8742615
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z18-994
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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