口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出 Detection of Kuchijirosho Associated Proteins by Western Blot with Sera of Tiger Puffer which Survived Kuchijirosho

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Kuchijirosho (snout ulcer disease) is an infectious disease of tiger puffer Takifugu rubripes in Japan and Korea. Little is known about detailed characteristics on the causative agent of kuchijirosho, except it is a filterable agent. In the present study, kuchijirosho associated proteins (KAPs) with molecular weight of 100-120 kDa were detected in brain tissues of tiger puffer by western blot analysis with sera of tiger puffer which survived kuchijirosho, but not in the kidney or spleen tissues. Interestingly, KAPs existed in brain tissues of tiger puffer with no history of kuchijirosho, however, the KAPs were not recognized by those healthy fish sera. KAPs were fractionated in supernatant of brain tissue homogenate containing the kuchijirosho agent by ultra-centrifugation, while pathogenicity of the kuchijirosho agent was observed in the precipitated fraction rather than the supernatant. These results suggest that KAPs are not structural proteins of the kuchijirosho agent, and could be brain tissue proteins antigenically altered by infection of the kuchijirosho agent. It is also considered that the causative agent could be low in antigenicity to host fish because structural proteins of the causative agent was never recognized by host fish sera.

Kuchijirosho (snout ulcer disease) is an infectious disease of tiger puffer <i>Takifugu rubripes</i> in Japan and Korea. Little is known about detailed characteristics on the causative agent of kuchijirosho, except it is a filterable agent. In the present study, kuchijirosho associated proteins (KAPs) with molecular weight of 100-120 kDa were detected in brain tissues of tiger puffer by western blot analysis with sera of tiger puffer which survived kuchijirosho, but not in the kidney or spleen tissues. Interestingly, KAPs existed in brain tissues of tiger puffer with no history of kuchijirosho, however, the KAPs were not recognized by those healthy fish sera. KAPs were fractionated in supernatant of brain tissue homogenate containing the kuchijirosho agent by ultra-centrifugation, while pathogenicity of the kuchijirosho agent was observed in the precipitated fraction rather than the supernatant. These results suggest that KAPs are not structural proteins of the kuchijirosho agent, and could be brain tissue proteins antigenically altered by infection of the kuchijirosho agent. It is also considered that the causative agent could be low in antigenicity to host fish because structural proteins of the causative agent was never recognized by host fish sera.

収録刊行物

  • 魚病研究  

    魚病研究 42(1), 29-34, 2007-03-15 

    日本魚病学会 = Japanese Society of Fish Pathology

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各種コード

  • NII論文ID(NAID)
    10018909311
  • NII書誌ID(NCID)
    AN00063165
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    0388788X
  • NDL 記事登録ID
    8742626
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z18-994
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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