Isolation of a homothallic mutant in <i>Lentinula edodes</i>
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- Shimomura Norihiro
- The Tottori Mycological Institute
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- Murakami Shigeyuki
- The Tottori Mycological Institute
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- Matsumoto Teruyuki
- The Tottori Mycological Institute
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- Maekawa Nitaro
- The Tottori Mycological Institute
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- Hasebe Kozaburo
- The Tottori Mycological Institute
Bibliographic Information
- Other Title
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- Isolation of a homothallic mutant in Lentinula edodes
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Abstract
<p>To obtain a homothallic mutant in Lentinula edodes, basidiospores derived from the common Bmut dikaryon (A1B1mut × A2B1mut) were treated with UV irradiation. Of a total of approximately 5000 monosporous cultures recovered, a single basidiospore isolate was found to produce the hyphae bearing clamp connections without mating. This mutant strain could form fruit bodies, and all its single basidiospore isolates developed into colonies with clamp connections. Such homothallic behaviors were transmitted from the mutant strain to the next generation. During the germination and following hyphal elongation in a single basidiospore of mutant strain, clamp connections were clearly detected in multicellular hyphae, which contained two nuclei in each cell. Their clamp connections were morphologically variable, viz., pseudo, abnormal, and true clamps. Amplified fragment length polymorphism (AFLP) profiles among the basidiospore isolates of mutant strain were identical, indicating that the mutant strain produced isogenic basidiospore progeny.</p>
Journal
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- Mycoscience
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Mycoscience 48 (2), 117-121, 2007
The Mycological Society of Japan
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Details 詳細情報について
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- CRID
- 1390295603315567744
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- NII Article ID
- 10018909746
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- NII Book ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL BIB ID
- 8706568
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Allowed