Integrated Rate Equation Considering Product Inhibition and Its Application to Kinetic Assay of Serum Ethanol

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著者

    • Liao Fei LIAO Fei
    • Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University
    • Zhao Li-na ZHAO Li-na
    • Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University
    • Zhao Yun-Sheng [他] ZHAO Yun-sheng
    • Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University
    • TAO Jia
    • Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University
    • ZUO Yuping
    • Department of Biochemistry & Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University

抄録

Kinetic assay of serum ethanol was investigated by predicting maximal product absorbance at 340 nm (<i>A</i><sub>mk</sub>) through fitting to the yeast alcohol dehydrogenase reaction curve with the integrated rate equation, taking into account product inhibition in the presence of semicarbazide. Predicted <i>A</i><sub>mk</sub> linearly responded to the preset constant of steady-state concentration of acetaldehyde (<i>C</i><sub>ald</sub>). An exponential correlation function was established between desired <i>C</i><sub>ald</sub> and putative <i>A</i><sub>mk</sub> for authentic ethanol. For unknown samples, iterative fitting to reaction curve till preset constant <i>C</i><sub>ald</sub> and resultant <i>A</i><sub>mk</sub> satisfied this exponential correlation function yielded <i>A</i><sub>mk</sub> with variation coefficient <4.3%. Variations in enzyme activity, data range and kinetic parameters showed negligible effects. The recovery was consistent to 100% with resistance to methanol and isopropanol. The upper limit of linear response for <i>A</i><sub>mk</sub> was about 40 times of the lower limit. These results indicated that this kinetic method was reliable for serum ethanol assays with obvious advantages.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 23(4), 439-444, 2007-04-10 

    The Japan Society for Analytical Chemistry

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各種コード

  • NII論文ID(NAID)
    10018914307
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    8698541
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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