Simultaneous Fluorometric Resolution of Pyridoxal, Pyridoxamine and 4-Pyridoxic Acid Using Chemometric Methods
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An alternatively minimizing covariant matrix error (AMCME) algorithm, newly proposed by the present authors, was applied to the simultaneous fluorometric determination of pyridoxal, pyridoxamine and 4-pyridoxic acid without loss of sensitivity. The experimental results illustrate that the profiles of spectra and concentration can be accurately resolved using the AMCME algorithm with a high sensitivity and stable repeatability. That is to say, the closely overlapping problem of the spectra could be resolved owing to the characteristic features of the AMCME algorithm.
- Analytical Sciences
Analytical Sciences 23(4), 453-455, 2007-04-10
The Japan Society for Analytical Chemistry