高圧力下における蛋白質結晶表面の分子ステップのその場観察 In Situ Observation of Molecular Steps on the Surface of Protein Crystal under High Pressure

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Velocities of elementary steps and two-dimensional nucleation rates on the {101} face of glucose isomerase (GI) crystals were measured at pressures of 0.1, 25 and 50 MPa. We conducted <i>in situ</i> observation of the steps by using a laser confocal microscope combined with differential interference contrast microscope (LCM-DIM) and high-pressure vessel with a sapphire window (t = 1 mm). Both step velocities and 2D nucleation rates increased with increasing pressure at <i>T</i> = 26.4°C and <i>C</i> = 5.6 mg ml<sup>-1</sup>. Solubilities C<sub>e</sub> at high pressures were also measured by <i>in</i> situ observation of the moving steps around equilibrium temperatures. The solubility decreased with increasing pressure.

収録刊行物

  • 高圧力の科学と技術 = The Review of high pressure science and technology  

    高圧力の科学と技術 = The Review of high pressure science and technology 17(1), 50-56, 2007-05-20 

    The Japan Society of High Pressure Science and Technology

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各種コード

  • NII論文ID(NAID)
    10018918449
  • NII書誌ID(NCID)
    AN10452913
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    0917639X
  • NDL 記事登録ID
    8742699
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z17-1589
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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