<i>Thermoanaerobacter brockii</i> Kojibiose Phosphorylaseによって合成された新規三および四糖の分離および同定

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  • Isolation and Identification of Novel Tri- and Tetra-saccharides Synthesized by Thermoanaerobacter brockii Kojibiose Phosphorylase
  • Isolation and identification of noble tri- and tetrasaccharides synthesized by Thermoanaerobacter brockii kojibiose phosphorylase

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Novel tri- and tetra-saccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate (β-D-G1P) to palatinose using Thermoanaerobacter brockii kojibiose phosphorylase. There saccharides were isolated using carbon-Celite column chromatography and preparative high performance liquid chromatography. Gas liquid chromatography analysis of methyl derivatives, MALDI-TOF MS and NMR measurements were used for structural confirmation of the saccharides. The 1H and 13C NMR signals of the saccharides were assigned using 2D-NMR including COSY, HSQC, HSQC-TOCSY and HMBC. These oligosaccharides were identified as 2G-α-D-glucopyranosyl-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→6)-D-fructofuranose and 2G(2-α-D-glucopyranosyl)2-palatinose; O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→2)-O-α-D-glucopyranosyl-(1→6)-D-fructofuranose.

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