グラム陰性菌感染症におけるリポ多糖抗原の免疫組織化学的検出  [in Japanese] Immunohistochemical Detection of Lipopolysaccharide in Gram-negative Bacterial Infection  [in Japanese]

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Author(s)

Abstract

病性鑑定材料のホルマリン固定パラフィン切片上で, グラム陰性菌を免疫組織化学的に識別できるか否かをリポ多糖 (LPS) に対する抗血清を用いて検討した. 抗LPS血清は検索したすべてのグラム陰性菌感染症例で交差陽性反応を示した. これらの症例では, 抗LPS血清を病変部の菌体や浸潤炎症性細胞の細胞質に陽性反応を示し, 各菌種に対する特異抗血清の反応性とほぼ一致していた. また, 一部の症例では菌種に特異的な抗血清に明瞭な反応のみられない壊死巣周囲の線維性肉芽組織中のマクロファージや巨細胞の細胞質にも, LPS血清の陽性反応が認められた. 本法によってパラフィン切片上の病変部でグラム陰性菌のLPS抗原を検出することができた.

Detection of a common antigen of Gram-negative bacteria was attempted with immunohistochemistry on formalin fixed paraffin sections. An anti-lipopolysaccharide (LPS) rabbit serum widely reacted with gramnegative bacterial antigens in the lesions. In addition, the anti-LPS serum was reactive with the bacterial antigens in the cytoplasm of infiltrated macrophages and giant cells in fibrous tissues where the species specific antisera were not reactive.

Journal

  • Journal of the Japan Veterinary Medical Association  

    Journal of the Japan Veterinary Medical Association 49(10), 714-719, 1996-10-20 

    Japan Veterinary Medical Association

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Codes

  • NII Article ID (NAID)
    10018940827
  • NII NACSIS-CAT ID (NCID)
    AN00191857
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    04466454
  • NDL Article ID
    4057972
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-352
  • Data Source
    CJP  NDL  IR  J-STAGE 
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