Responses of Dot-Immunobinding and Agglutinating Antibodies in Chickens Infected with Mycoplasmas

• TAKAHATA Toshihiro

  Animal Health Products and Chemicals Research Center

• TAKEI Masakazu

  Animal Health Products and Chemicals Research Center

• KATO Masahiro

  Animal Health Products and Chemicals Research Center

• SHIMIZU Takamasa

  宮崎大学農学部

Bibliographic Information

Other Title

• マイコプラズマ感染鶏におけるDot免疫結合抗体と凝集抗体の推移
• マイコプラズマ カンセン ニワトリ ニ オケル Dot メンエキ ケツゴウ コ

Abstract

Eleven SPF White Leghorn female chickens were inoculated intratracheally with Mycoplasma gallisepticum, while 11 chickens withM. synoviae. Sera were examined for dot-immunobinding (DIB) and agglutinating antibodies every week for 10 weeks after inoculation. All sera from inoculated chickens were shown to have high positivity against the inoculated organisms in both tests, whereas sera from uninoculated control chickens were negative. In agglutination test remarkable false-positive reaction was observed with sera from both inoculated groups. In the DIB test, however, non-specific reaction was never observed. Results of the DIB test with sera at their 100-fold dilution coincided with the results of agglutination test at a level of 86% with MG and 92% with MS antigen.

Journal

• Journal of the Japan Veterinary Medical Association

  Journal of the Japan Veterinary Medical Association 49 (8), 533-535, 1996

  Japan Veterinary Medical Association

Keywords

• dot-immunobinding
• mycoplasma
• non-specific agglutination

Details

• CRID: 1390001204707848192
• NII Article ID: 10018941250
• NII Book ID: AN00191857
• DOI: 10.12935/jvma1951.49.533
• ISSN: 21860211
• ISSN: 04466454
• ISSN: http://id.crossref.org/issn/87507943
• NDL BIB ID: 4021832
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010542680
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4021832
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed