豚の血液および脾臓単核球からの日本脳炎ウイルスゲノムの検出  [in Japanese] Detection of Japanese Encephalitis Virus Genome in Mononuclear Cells from Blood and Spleen of Swine  [in Japanese]

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Abstract

豚 (6~7ヵ月齢) における日本脳炎 (JE) ウイルスの持続感染の可能性を明らかにする目的で, 血液と脾臓からNested PCRによりJEウイルスゲノムの検出を試みた. 金沢市で1993年9月~1994年2月に調べた豚50頭中血液陽性検体は, 9月に10例中4例, 10月に11例中2例, 11月に9例中2例, 12月に10列中3例であった. 2月の10例の血液からはウイルスゲノムを検出できなかったが, 3例の脾臓単核球からウイルスゲノムが増幅された. いっぽう, 香川県では, 1995年7月~1996年3月に採取した豚160頭の血液について検索したところ, 陽性血液検体は9月に5例, 10月に2例, 11月に1例で, 1月, 2月, 3月の各20例からはウイルスゲノムが検出されなかったが, 脾臓単核球からは1月に1例, 2月に2例, 3月に1例でウイルスゲノムが増幅された. 以上の成績から, 冬期間にJEウイルスが豚の血液あるいは脾臓単核球に持続感染している可能性が示唆された.

Possible persistent infection of Japanese encephalitis (JE) virus was investigated by detecting the virus genome using nested PCR from the blood or spleen of 6 to 7-month-old swine. In Kanazawa city, blood mononuclear cells were collected from 50 cases in September 1993 to February 1994, and 4 of 10 in September, 2 of 11 in October, 2 of 9 in November, 3 of 10 in December, were positive for the virus genome. In February 1994, the virus genome was amplified in splenic mononuclear cell samples of 3 of 10 cases, which were negative for virus genome in blood mononuclear cell samples. On the other hand, in Kagawa Precfecture where 160 cases were examined from July 1995 to March 1996, the virus genome was detectable from the blood samples in 5 of 20, 2 of 20 and one of 20 in September, October, and November, respectively. The virus genome was detected in January, February and March in spleen mononuclear cells from 4 cases, which were negative in the blood samples. These results suggested that JE virus persistently infected the mononuclear cells of blood and spleen of swine during the winter period.

Journal

  • Journal of the Japan Veterinary Medical Association  

    Journal of the Japan Veterinary Medical Association 50(12), 731-734, 1997-12-20 

    Japan Veterinary Medical Association

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Codes

  • NII Article ID (NAID)
    10018949908
  • NII NACSIS-CAT ID (NCID)
    AN00191857
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    04466454
  • NDL Article ID
    4381391
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-352
  • Data Source
    CJP  NDL  IR  J-STAGE 
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