Detection of Verocytotoxin-Producing <I>Escherishia coli</I> from Cattle Feces by PCR Using Pooled Samples

• YOKOKOJI Kiyoyuki

  Chiran Meat Inspection Center of Kagoshima Prefecture

• FUJIMOTO Hideki

  Chiran Meat Inspection Center of Kagoshima Prefecture

• UCHIZONO Seiya

  Chiran Meat Inspection Center of Kagoshima Prefecture

• CHUMA Takehisa

  鹿児島大学農学部

• OKAMOTO Karoku

  鹿児島大学農学部

Bibliographic Information

Other Title

• PCR法による牛直腸内容集合検体からのVero毒素産生性大腸菌遺伝子の検出
• PCRホウ ニヨル ウシ チョクチョウ ナイヨウ シュウゴウ ケンタイ カラ
• Detection of Verocytotoxin-Producing Escherishia coli from Cattle Feces by PCR Using Pooled Samples

Abstract

From May to October 1995 in Kagoshima Prefecture, the rectal contents of 100 slaughtered healthy cattle were inoculated onto the SIB agar, and pooled samples of IPA-negative colonies produced were surveyed by PCR for verocytotoxin genes. The genes were readily detected from 12 of rectal content, and the detection of verocytotoxin-producing Escherichia coli was efficiently performed with the following serological and biochemical examination.

Journal

• Journal of the Japan Veterinary Medical Association

  Journal of the Japan Veterinary Medical Association 50 (10), 607-610, 1997

  Japan Veterinary Medical Association

Keywords

• cattle
• PCR
• rectal content
• VTEC

Details

• CRID: 1390282679685864192
• NII Article ID: 10018950154
• NII Book ID: AN00191857
• DOI: 10.12935/jvma1951.50.607
• ISSN: 21860211
• ISSN: 04466454
• NDL BIB ID: 4335257
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030562115
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4335257
• Web Site: https://search.jamas.or.jp/link/ui/1998043803
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed