遺伝子組み換えヒト骨形成因子によるラット下腿筋肉内骨誘導におけるニコチンの影響 Effect of nicotine on osteoinduction by recombinant human bone morphogenetic protein-2

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著者

    • 大久保 康則 [他] OKUBO Yasunori
    • 京都大学大学院医学研究科感覚運動系外科学講座口腔外科学分野 Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University
    • 河井 まりこ KAWAI Mariko
    • 岡山大学大学院医歯学総合研究科生体機能再生・再建学講座口腔形態学分野 Oral Morphology, Department of Science of Functional Recovery and Reconstruction, Graduate School of Medicine and Dentistry, Okayama University
    • 別所 和久 BESSHO Kazuhisa
    • 京都大学大学院医学研究科感覚運動系外科学講座口腔外科学分野 Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University

抄録

We evaluated the effect of nicotine on osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2). Five micrograms of rhBMP-2 was implanted into the calf muscle of 10-week-old Wistar rats. For 14 days after implantation, nicotine was continuously administered subcutaneously in a dose of 0.1 (group L), 1.0 (group M), or 10.0 (group H) mg/kg/day. In the control group (group C), only physiological saline was administered. On day 21 after implantation, soft radiographs were obtained, and histological and biochemical analyses were performed. The results suggested that a high dose of nicotine pre ents bone formation.<BR>In addition, we investigated the effect of nicotine on osteoinduction. For 14 days after rhBMP-2 implantation, nicotine was continuously administered in the dose used in group M. The control group was given only physiological saline. On days 3, 7, 14, and 21 after implantation, soft radiographs were obtained after the injection of contrast media, and histological and biochemical analyses were performed. In the radiographic study with contrast media, there were fewer small capillaries in the rhBMP-2-implanted muscle in the nicotine group than in the control group. Histologically, nicotine reduced new bone and cartilage formation and decreased the number of capillaries. On quantitative biochemical analysis, indices of bone formation in the nicotine group were significantly lower than those in control. These results suggest that the prevention of bone formation by nicotine may be caused by a reduced blood supply to rhBMP-2-implanted muscle.

収録刊行物

  • 日本口腔外科学会雑誌  

    日本口腔外科学会雑誌 51(6), 274-279, 2005-06-20 

    Japanese Society of Oral and Maxillofacial Surgeons

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各種コード

  • NII論文ID(NAID)
    10018954979
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • NDL 記事登録ID
    7403508
  • NDL 雑誌分類
    ZS44(科学技術--医学--歯科学・口腔外科学)
  • NDL 請求記号
    Z19-145
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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