Medium-term Rat Liver Bioassay for Rapid Detection of Hepatocarcinogenic Substances.
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- 伊東 信行
- Nagoya City University
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- 長谷川 良平
- First Department of Pathology, Nagoya City University Medical School
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- 今井田 克己
- First Department of Pathology, Nagoya City University Medical School
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- 広瀬 雅雄
- First Department of Pathology, Nagoya City University Medical School
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- 白井 智之
- First Department of Pathology, Nagoya City University Medical School
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- 玉野 静光
- First Department of Pathology, Nagoya City University Medical School Daiyukai Institute of Medical Science
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- 萩原 昭裕
- First Department of Pathology, Nagoya City University Medical School Daiyukai Institute of Medical Science
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抄録
We have developed a medium-term liver bioassay system for rapid detection of carcinogenic agents using male F344 rats to bridge the gap between long-term carcinogenicity tests and short-term screening assays. The system is fundamen-tally based on the two-stage hypothesis with regard to tumor production, employing initiation by diethylnitrosamine (200mg/kg, ip) in the first stage and test chemical administration during the second, in combination with 2/3 partial hepatectomy. It requires only 8 weeks for animal experimentation and a further few weeks for quantitative analysis of immunohistochemically-demonstrated glutathione S-transferase placental form positive hepatic foci. A total of 277 chemicals/substances have already been analyzed in our laboratory and the efficacy of the system for hepatocarcinogens has thereby been well established. This bioassay is increasingly becoming regarded as an appropriate alternative test system for carcinogenicity risk assessment. It is particularly useful for studies which require many experimental groups and for evaluation of chemopreventive agents.
収録刊行物
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 10 (1), 1-11, 1997
日本毒性病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204416289152
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- NII論文ID
- 10019087001
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- NII書誌ID
- AA12022786
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- DOI
- 10.1293/tox.10.1
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- COI
- 1:CAS:528:DyaK1cXktlWlsbk%3D
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- ISSN
- 1881915X
- 09149198
- http://id.crossref.org/issn/09149198
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可