ウナギ好中球の respiratory burst

• 飯田 貴次

  Department of Animal, Grassland and Fishery Sciences, Faculty of Agriculture, Miyazaki University

• 若林 久嗣

  Department of Fisheries, Faculty of Agriculture, The University of Tokyo

書誌事項

タイトル別名

• Respiratory Burst of Japanese Eel Nutrophils.
• ウナギ好中球のrespiratory burst〔英文〕
• ウナギ コウチュウキュウ ノ respiratory burst エイブン

抄録

Japanese eel (Anguilla japonica) neutrophils underwent a striking increase in oxygen consumption by stimulation, which is usually called respiratory burst. This increased uptake of oxygen was insensitive to mitochondrial respiration inhibitor such as potassium cyanide. Superoxide was detected during the respiratory burst by measuring chemiluminescence (CL) enhanced by 2-methyl-6-phenyl-3, 7-dihydroimidazo [1, 2-a] pyrazine-3-one (CLA) which is highly specific and sensitive to superoxide. EDTA blocked while EGTA stilumated CLA-enhanced CL. These characters of the respiratory burst suggest that Japanese eel neutrophils have NADPH oxidase-like activity similar to the mammalian NADPH oxidase. Sodium azide, an inhibitor of myeloperoxidase (MPO) activity, did not affect luminol-enhanced CL, which is thought to be dependent on hypochlorous acid (HOCl) produced by catalysis of MPO. It is suggested that Japanese eel neutrophils do not generate HOCl during the respiratory burst because of lacking MPO in cytoplasm.

収録刊行物

• 魚病研究

  魚病研究 30 (4), 257-261, 1995

  日本魚病学会

キーワード

• respiratory burst
• Japanese eel
• Anguilla japonica
• neutrophil
• phagocytosis
• active oxygen
• chemiluminescence

詳細情報

• CRID: 1390282681438689792
• NII論文ID: 10019209571
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.30.257
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 3644344
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3644344
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可