イムノゴールド法の伝染性膵臓壊死症ウイルス(IPNV)の電顕観察への応用

  • Novoa B.
    Instituto de Investigaciones Marinas. CSIC.

書誌事項

タイトル別名
  • Immunogold Technique Applied to Electron Microscopy of Infectious Pancreatic Necrosis Virus(IPNV).
  • Immunogold technique applied to electro

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抄録

Immunogold technique in electron microscopy proved to be effective to demonstrate the virusantibody specificity using purified reference strains of infectious pancreatic necrosis virus (IPNV). Although some cross reactions were detected, it was possible to distinguish the major IPNV serotypes (Sp, Ab and WB) according to the different affinity for the antisera against the three reference strains which were binded to protein A-gold particles. Moreover, tubular structures with a diameter of 60 nm were detected presenting a high affinity for the gold particles, which suggests a viral origin.

収録刊行物

  • 魚病研究

    魚病研究 31 (3), 141-143, 1996

    日本魚病学会

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