酵素電気泳動法, カタラーゼテストおよび PCR-RFLP 解析による Edwardsiella tarda の型別

• 山田 義行

  Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• 若林 久嗣

  Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

書誌事項

タイトル別名

• Enzyme Electrophoresis, Catalase Test and PCR-RFLP Analysis for the Typing of Edwardsiella tarda.
• Enzyme electrophoresis catalase test an

抄録

Enzyme electrophoresis for superoxide dismutase (SOD) and catalase divided 144 Edwardsiella tarda strains into two groups; group 1 contained all the strains isolated from diseased fish in Japan and group 2 included 25 strains from non-disease sources and reference strain ATCC15947^T. These groups could be similarly distinguished by a simple catalase test; group 1 showed an instantaneous bubbling, whereas in group 2 bubbling was delayed or weak. PCR-RFLP analysis usingHhaI revealed that the two groups differed in their 16S rDNA genotypes. Furthermore, this analysis subdivided group 1 into two genotypes.

収録刊行物

• 魚病研究

  魚病研究 33 (1), 1-5, 1998

  日本魚病学会

キーワード

• Edwardsiella tarda
• SOD
• catalase
• 16S rDNA
• enzyme electrophoresis
• PCR
• RFLP

詳細情報

• CRID: 1390282681436996224
• NII論文ID: 10019210726
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.33.1
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 4425460
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4425460
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可