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- 柳田 哲也
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Freeman Mark A.
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- 野村 昌功
- Kumamoto Prefectural Fisheries Research Center
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- 高見 生雄
- Nagasaki Prefectural Institute of Fisheries
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- 杉原 志貴
- Nagasaki Prefectural Institute of Fisheries
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- 横山 博
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- 小川 和夫
- Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
書誌事項
- タイトル別名
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- Development of a PCR-based Method for the Detection of Enteric Myxozoans Causing the Emaciation Disease of Cultured Tiger Puffer
この論文をさがす
抄録
A polymerase chain reaction (PCR) -based method was developed to detect enteric myxozoans which cause the emaciation disease of cultured tiger puffer Takifugu rubripes. Three primer sets were designed based on the sequences of small subunit ribosomal DNA of Enteromyxum leei, Enteromyxum fugu and Leptotheca fugu. All of the primer sets specifically amplified the target DNA of each species. The PCR successfully detected parasite DNA not only from the intestinal mucosa of killed fish but also from gut contents collected from live fish using a swab inserted into the anus. These PCR analyses were more sensitive to detection of parasites than microscopic observation on the intestinal imprints. The present PCR method is useful for rapid diagnosis of the myxosporean emaciation disease.
収録刊行物
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- 魚病研究
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魚病研究 40 (1), 23-28, 2005
日本魚病学会
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キーワード
詳細情報
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- CRID
- 1390001206461896320
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- NII論文ID
- 10019225072
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- NII書誌ID
- AN00063165
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- COI
- 1:CAS:528:DC%2BD2MXkt1Wjtr0%3D
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- ISSN
- 18817335
- 0388788X
- http://id.crossref.org/issn/0388788X
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- NDL書誌ID
- 8054951
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可