Elevated Serum Bisphenol A Levels under Hyperandrogenic Conditions may be Caused by Decreased UDP-glucuronosyltransferase Activity

• TAKEUCHI Toru

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• TSUTSUMI Osamu

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• IKEZUKI Yumiko

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• KAMEI Yoshimasa

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• OSUGA Yutaka

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• FUJIWARA Toshihiro

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• TAKAI Yasushi

  Department of Obstetrics and Gynecology, Saitama Medical Center

• MOMOEDA Mikio

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• YANO Tetsu

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

• TAKETANI Yuji

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

抄録

This study was performed to investigate the effect of androgen on the metabolism of bisphenol A (BPA), an endocrine disruptor, in order to clarify the mechanism of the higher levels of serum BPA in men and hyperandrogenemic women compared with normal women. Castrated female rats (OVX) were subcutaneously injected with testosterone propionate (TP) (0.01, 0.1, and 1 mg) every day for 2 weeks. Serum BPA concentrations in OVX rats showed a TP dose-dependent increase and were significantly higher at 0.1 and 1.0 mg of TP. The enzyme reaction of BPA glucuronidation in the rat liver microsomes showed that the ratio of glucuronide in the OVX rats was significantly reduced in a TP dose-dependent manner. Analysis of the mRNA expression of UDP-glucuronosyltransferase 2B1 (UGT2B1) by real-time quantitative RT-PCR revealed that the relative expression level of UGT2B1 mRNA showed a TP dose-dependent decrease. The results of enzyme analyses demonstrated that the ratio of BPA glucuronidation and the expression level of UGT2B1 mRNA were significantly lower under the hyperandrogenemic conditions. The clearance of BPA may be slowed in a TP dose-dependent manner, resulting in an increase of serum BPA concentration under hyperandrogenemic conditions.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 53 (4), 485-491, 2006

  一般社団法人 日本内分泌学会

キーワード

• Bisphenol A
• UDP-glucuronosyltransferase
• UGT2B1
• Endocrine disruptor
• Androgen

詳細情報

• CRID: 1390001206300733696
• NII論文ID: 10019269724
• NII論文ID: 130004443249
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.k06-032
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/53/4/53_4_485/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007071202
• 本文言語コード: en
• データソース種別:
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  • KAKEN
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