Evaluating promoter sequences for trapping an enhancer activity in the silkworm Bombyx mori

DOI Web Site 被引用文献3件 参考文献23件
  • Uchino Keiro
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Imamura Morikazu
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Sezutsu Hideki
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Kobayashi Isao
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Kojima Katsura
    Silk-materials Research Unit, National Institute of Agrobiological Sciences
  • Kanda Toshio
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Tamura Toshiki
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences

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  • Evaluating Promoter Sequences for Trapping an Enhancer Activity in the Silkworm <i>Bombyx mori</i>

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To investigate the effect of a promoter sequence for detecting enhancer activity in the silkworm Bombyx mori, we constructed six different GAL4 vectors under the control of different minimum promoters. In addition, a homozygous UASGFP strain was constructed and used to test expression of the GAL4 gene. Green fluorescent protein (GFP) expression was compared in the transgenic strains with the different GAL4 gene inserts. The most prominent difference in the expression pattern in the different strains was observed when the Bombyx cytoplasmic actin gene (BmA3) promoter was used. We concluded that using the GAL4 gene with the BmA3 promoter would be the most useful way for detecting the positional effect of genes inserted in the silkworm.<br>

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