Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ｓｈｉｎｓｈｕｅｎｓｅとＭｙｃｏｂａｃｔｅｒｉｕｍ　ｌｅｐｒａｅの分子生物学的診断法の有用性

DOI PubMed 被引用文献1件 参考文献14件
  • 中永 和枝
    国立感染症研究所ハンセン病研究センター生体防御部
  • 鈴木 幸一
    国立感染症研究所ハンセン病研究センター生体防御部
  • 谷川 和也
    国立感染症研究所ハンセン病研究センター生体防御部
  • 岩本 朋忠
    神戸市環境保健研究所微生物部
  • 後藤 正道
    鹿児島大学大学院医歯学総合研究科人体がん病理学
  • 斎藤 肇
    広島県環境保健協会
  • 石井 則久
    国立感染症研究所ハンセン病研究センター生体防御部

書誌事項

タイトル別名
  • Mycobacterium shinshuense and Mycobacterium leprae infections: usefulness of genetical examinations

この論文をさがす

抄録

Although Mycobacterium shinshuense and M. leprae infections are relatively rare in the fields of dermatology, an early diagnosis is one of the important prognostic factors of these infections. Applications of the genetical examinations such as PCR and 16S rDNA sequencing are helpful in early diagnosis with culture nagative cases. Short target PCR tests are available to detect DNA of M. shinshuense or M. leprae from clinical specimens including formalin fixed-paraffin embedded samples. A partial 16s rDNA sequencing is functional with enough intact bacterial DNA. A similarity search based on the partial 16S rDNA sequences using RIDOM database is an easy and powerful tool to estimate the species of mycobacteria, however, it is not enough for the identification in some cases. For instance, a clinical isolate of M. shinshuense is clearly discriminated from M. leprae (92.75% sequence identity), however, difficult to be identified from M. marinum and M. ulcerans (99.77% sequence identity). The phylogenetic tree based on 16S rDNA sequences is illustrating that both M. leprae (closely related to M. haemophilum) and M. shinshuense (closely related to M. marimum and M. ulcerans, and also M. tuberculosis) are relatively related species and distantly related to rapidly growing species among 30 species of pathogenic mycobacteria which have been isolated in Japan.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (14)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ