余剰活性汚泥の高温嫌気性消化における微生物群集の構造及び多様性の解析

• 小林 拓朗

  東北大学大学院工学研究科土木工学専攻

• 李 玉友

  東北大学大学院工学研究科土木工学専攻

• 原田 秀樹

  東北大学大学院工学研究科土木工学専攻

書誌事項

タイトル別名

• Characterization of Microbial Community Structure and Diversity, Present in Thermophilic Anaerobic Digestion of Waste-Activated Sludge
• ヨジョウ カッセイ オデイ ノ コウオン ケンキセイ ショウカ ニ オケル ビセイブツ グンシュウ ノ コウゾウ オヨビ タヨウセイ ノ カイセキ

抄録

The microbial community structures present in the thermophilic anaerobic digestion (TAD) of waste-activated sludge (WAS) and WAS were analyzed with molecular biological techniques including polymerase chain reaction-denature gradient gel electrophoresis (PCR-DGGE), real-time PCR, and cloning analysis. No significant differences in the microbial community structures present in TAD were observed among three reactors operated with hydraulic retention times of 10, 20 and 30 days. The microbial community present in TAD had less diversity than that present in WAS, and the sequences obtained in WAS were not present in TAD by the cloning analysis. In the TAD bacterial clone library, 97.5% of the total clones belonged to Firmicutes and 73.1% belonged to Coprothermobacter. Real-time PCR and cloning analyses revealed that the number of Methanosarcina thermophila, which is an acetoclastic methanogen, was larger than that of Methanoculleus thermophilus, which is a hydrogenotrophic methanogen, in terms of the numbers of copies of 16S rDNA.

収録刊行物

• 水環境学会誌

  水環境学会誌 30 (7), 369-375, 2007

  公益社団法人 日本水環境学会

キーワード

• anaerobic digestion
• thermophilic
• waste-activated sludge
• molecular biological analysis

詳細情報

• CRID: 1390001205082011776
• NII論文ID: 10019520258
• NII書誌ID: AN10372439
• DOI: 10.2965/jswe.30.369
• COI: 1:CAS:528:DC%2BD2sXptVaqsLk%3D
• ISSN: 18813690
• ISSN: 09168958
• NDL書誌ID: 8888870
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8888870
• Web Site: https://www.jstage.jst.go.jp/article/jswe/30/7/30_7_369/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可