Development of compound microsatellite markers in the toxic dinoflagellate <i>Alexandrium catenella</i> (Dinophyceae)
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- NISHITANI GOH
- Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
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- NAGAI SATOSHI
- Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
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- MASSERET ESTELLE
- University Montpellier 2, Laboratoire Ecosystèmes Lagunaires (UMR-CNRS 5119, ECOLAG), cc93
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- LIAN CHUNLIAN
- Asian Natural Environmental Science Center, the University of Tokyo
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- YAMAGUCHI SANAE
- Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
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- YASUDA NINA
- Graduate School of Information Science and Engineering, Tokyo Institute of Technology
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- ITAKURA SHIGERU
- Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
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- GRZEBYK DANIEL
- University Montpellier 2, Laboratoire Ecosystèmes Lagunaires (UMR-CNRS 5119, ECOLAG), cc93
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- BERREBI PATRICK
- University Montpellier 2, Institut des Sciences de l'Evolution (UMR-CNRS 5554), cc065
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- SEKINO MASASHI
- Tohoku National Fisheries Research Institute
Bibliographic Information
- Other Title
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- Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)
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Abstract
In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species.
Journal
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- Plankton and Benthos Research
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Plankton and Benthos Research 2 (3), 128-133, 2007
The Plankton Society of Japan, The Japanese Association of Benthology
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Details 詳細情報について
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- CRID
- 1390001205265843712
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- NII Article ID
- 10019844941
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- NII Book ID
- AA12130745
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- ISSN
- 1882627X
- 18808247
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- NDL BIB ID
- 8881062
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed