Radical Scavenging Activity and Inhibition of Macrophage NO Production by Fukinolic Acid, a Main Phenolic Constituent in Japanese Butterbur (Petasites japonicus)

• WATANABE Satoru

  Department of Health and Nutritional Sciences, Tokyo Seiei College

• HASHIMOTO Ken

  Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry

• TAZAKI Hiroyuki

  Department of Veterinary and Animal Science, Nippon Veterinary and Life Science University

• IWAMOTO Yuzuri

  Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government

• SHINOHARA Naoko

  Department of Health and Nutritional Sciences, Tokyo Seiei College

• SATOH Kazue

  Department of Anatomy, School of Medicine, Showa University

• SAKAGAMI Hiroshi

  Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry

抄録

Four phenolic compounds were isolated from Japanese butterbur (Petasites japonicus) by preparative RP-HPLC technique, and were identified as chlorogenic acid (CGA), fukinolic acid (FA), 3,5-dicaffeoyl quinic acid, and 3,4,5-tricaffeoyl quinic acid. Since the antioxidant activity of FA among these four compounds has not been reported in detail, we investigated the scavenging activity of FA against several active oxygens and radicals, compared with CGA, gallic acid (GA) and epigallocatechin gallate (EGCG), using ESR spectroscopy. The order of potency of scavenging superoxide anion, nitric oxide (NO) and DPPH was EGCG>FA>CGA. The order of potency of scavenging hydroxyl radical was EGCG>FA>GA. FA inhibited the lipopolysaccharide-stimulated NO production and inducible NO synthase protein and mRNA expression by mouse macrophage-like cells Raw 264.7 more potently than CGA. The present study suggests the usefulness of FA as an antioxidant.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 13 (4), 366-371, 2007

  公益社団法人 日本食品科学工学会

キーワード

• fukinolic acid
• scavenging activity
• ESR
• NO
• inducible NO synthase

詳細情報

• CRID: 1390001204454327808
• NII論文ID: 10019973025
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.13.366
• COI: 1:CAS:528:DC%2BD1cXitVGmt70%3D
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可