天然保存料カワラヨモギ抽出物の抗菌活性成分  [in Japanese] Antimicrobial Activity and Constituents in Rumput Roman Extract as a Natural Food Preservative  [in Japanese]

天然保存料であるカワラヨモギ抽出物の成分組成を明らかとする目的で，本抽出物製品についてGC/MS分析を行い，さらに，主要な5成分については，単離精製し，NMRで化学構造を確認した結果，capillin, capillene, caryophyllene oxide, α-curcumene, methyleugenolであることを明らかとした．次に，ハロー試験法により，E. coli, S. cerevisiae およびA. niger に対する抗菌活性について検討した結果，本抽出物製品の活性本体がcapillinであることが確認された．HPLCにより，本抽出物製品中のcapillinおよびcapilleneの定量を行った結果，今回分析した製品中にはそれぞれ17.9 mg/mL, 36.1 mg/mL含有されることを明らかとした．

Rumput roman extract is used as a natural food preservative. Its antimicrobial activity and constituents were investigated as part of an ongoing study to evaluate its quality and safety as a food additive. The constituents were analyzed by GC/MS, and 5 major constituents were isolated and identified as capillin, capillene, caryophyllene oxide, α-curcumene and methyleugenol using NMR analysis. The antimicrobial activities against E. coli, S. cerevisiae and A. niger were measured by means of the halo test. Based on the results, we confirmed that capillin was the major active constituent. The concentrations of capillin and capillene were determined to 17.9 mg/mL and 36.1 mg/mL, respectively, from standard curves of authentic compounds on HPLC.