ヒラメのβ溶血性レンサ球菌症に対するファージ治療試験

• 松岡 学

  愛媛県中予水産試験場

• 橋爪 貴也

  広島大学大学院生物圏科学研究科

• 神崎 博幸

  佐賀県玄海水産振興センター

• 岩本 恵美

  広島大学大学院生物圏科学研究科

• Se Chang Park

  College of Veterinary Medicine, Seoul National University

• 吉田 照豊

  宮崎大学農学部

• 中井 敏博

  広島大学大学院生物圏科学研究科

書誌事項

タイトル別名

• Phage Therapy against .BETA.-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus
• ヒラメ ノ ベータ ヨウケツセイ レンサ キュウキンショウ ニ タイスル ファージ チリョウ シケン
• Phage Therapy against β-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus

抄録

We examined the therapeutic effect of Streptococcus iniae phages isolated from fish culture environments against experimental streptococcicosis of Japanese flounder Paralichthys olivaceus. Phage sensitivity tests with a double agar method revealed that 31 of 35 S. iniae strains from the flounder have a similar sensitivity to six phage isolates. In phage therapy experiments, fish were injected intraperitoneally (IP) with S. iniae PSi402 and 1 h later IP-injected with a mixture of two or four phage isolates, and observed at 25°C for 2 wk. Mortalities of fish receiving phages were significantly lower than those of control fish without phage-treatment in all four trials. The effect of phage treatment was also demonstrated even at 24 h post-infection, when cell numbers of S. iniae were 10^7.4 and 10^4.5 CFU/g in the kidneys and brains of fish, respectively. However, as phage-resistant S. iniae were frequently isolated from dead fish in the phage-treated group, further investigations are required to establish phage therapy of the disease.

収録刊行物

• 魚病研究

  魚病研究 42 (4), 181-189, 2007

  日本魚病学会

キーワード

• bacteriophages
• phage therapy
• <i>β</i>-hemolytic streptococcicosis
• <i>Streptococcus iniae</i>
• <i>Paralichthys olivaceus</i>
• Japanese flounder

詳細情報

• CRID: 1390282681437497728
• NII論文ID: 10020025016
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.42.181
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 9318255
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00028691
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9318255
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可