Identification and characterization of a second, inducible promoter of relA in Escherichia coli

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  • Nakagawa Akira
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • Oshima Taku
    Graduate School of Information Sciences, Nara Institute of Science and Technology
  • Mori Hirotada
    Graduate School of Biological Sciences, Nara Institute of Science and Technology

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The alarmone ppGpp is an important signal molecule for the stringent response. Escherichia coli relA encodes a ppGpp synthetase, and although the regulation of RelA protein activity has been studied extensively, the regulation of relA transcription remains unclear. Here, we describe a novel relA promoter, relAP2. According to quantitative measurement of mRNA by primer extension analysis, the previously reported promoter relAP1 is constitutively active throughout growth, while relAP2 is induced temporarily at the transition state between the exponential growth and stationary phases. A chromosomal transcriptional lacZ fusion (relAP2-lacZ) showed that relAP2 is positively regulated by H-NS and CRP. Furthermore, the reduced activity of relAP2-lacZ in an hns mutant could be rescued by an rpoS mutation, which is sufficient to derepress the relAP2-lacZ activity. These data suggest that transient expression from the relAP2 promoter is controlled by several global regulators. This may account for the complex regulation of relA expression in Escherichia coli.<br>

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