Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of shallot (<italic>Allium cepa</italic> L.)

• Masuzaki Shin-ichi

  The United Graduate School of Agricultural Sciences, Tottori University Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University

• Shigyo Masayoshi

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University

• Yamauchi Naoki

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University

書誌事項

タイトル別名

• Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.)

抄録

We analyzed Japanese bunching onion (Allium fistulosum L.) – shallot (Allium cepa L. Aggregatum group) alien chromosome addition lines in order to assign the genes involved in the flavonoid biosynthesis pathway to chromosomes of the shallot. Two complete sets of alien monosomic additions (2n = 2x + 1 = 17) were used for determining the chromosomal locations of several partial sequences of candidate genes, CHS, CHI, F3H, DFR, and ANS via analyses of PCR-based markers. The results of DNA marker analyses showed that the CHS-A, CHS-B, CHI, F3H, DFR, and ANS genes should be assigned to chromosomes 2A, 4A, 3A, 3A, 7A, and 4A, respectively. HPLC analyses of 14 A. fistulosum – shallot multiple alien additions (2n = 2x + 2 – 2x + 7 = 18 – 23) were conducted to identify the anthocyanin compounds produced in the scaly leaves. A direct comparison between the genomic constitution and the anthocyanin compositions of the multiple additions revealed that a 3GT gene for glucosylation of anthocyanidin was located on 4A. Thus, we were able to assign all structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of A. cepa.<br>

収録刊行物

• Genes & Genetic Systems

  Genes & Genetic Systems 81 (4), 255-263, 2006

  日本遺伝学会

キーワード

• alien chromosome addition line
• <i>Allium cepa</i>
• <i>Allium fistulosum</i>
• chromosomal location
• flavonoid biosynthetic gene

詳細情報

• CRID: 1390282680450756736
• NII論文ID: 10020145469
• NII書誌ID: AA11077421
• DOI: 10.1266/ggs.81.255
• ISSN: 18805779
• ISSN: 13417568
• NDL書誌ID: 8070822
• Web Site: https://petit.lib.yamaguchi-u.ac.jp/417
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8070822
• Web Site: http://www.jstage.jst.go.jp/article/ggs/81/4/81_4_255/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可