Hydrogen Peroxide-Induced Thymidine Incorporation Into Cultured Rat Astrocytes

• Tanaka Koh-ichi

  Department of Applied Pharmacology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan

• Sato Tomoaki

  Department of Applied Pharmacology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan

• Ohnishi Yoshiko

  Department of Applied Pharmacology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan

• Nishikawa Takashige

  Department of Applied Pharmacology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan

抄録

We characterized [methyl-³H]thymidine ([³H]thymidine) and [5-³H]uridine ([³H]uridine) incorporation into cultured astrocytes and neurons in the presence and absence of hydrogen peroxide (H₂O₂) in order to define the response to oxidative stress in the central nervous system. [³H]Thymidine incorporation into cultured astrocytes was remarkably decreased by N⁶,2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP), a permeable analogue of cAMP, which induced a morphological change from the polygonal form (undifferentiated astrocytes) to the process-bearing one (differentiated astrocytes). H₂O₂ induced [³H]thymidine, but not [³H]uridine, incorporation into cultured astrocytes at only an early time from 24 h after DBcAMP treatment, although the absolute quantities of [³H]thymidine incorporation into astrocytes pretreated with DBcAMP were less than those into astrocytes pretreated without DBcAMP. Hydroxyurea, a replicative DNA synthesis inhibitor, suppressed dose-dependently and completely [³H]thymidine incorporation into astrocytes pretreated without DBcAMP, but not astrocytes pretreated with DBcAMP. H₂O₂ did not stimulate [³H]thymidine or [³H]uridine incorporation into astrocytes pretreated without DBcAMP and neurons. These findings indicate that only astrocytes pretreated with DBcAMP are able to increase thymidine incorporation specifically in the presence of H₂O₂ for a purpose other than proliferation, including the repair of H₂O₂-induced DNA injury, for example.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 102 (3), 296-304, 2006

  公益社団法人 日本薬理学会

キーワード

• hydrogen peroxide (H₂O₂)
• thymidine incorporation
• astrocyte
• <i>N</i>⁶,2'-<i>O</i>-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP)
• hydroxyurea

詳細情報

• CRID: 1390282680152850304
• NII論文ID: 10020345080
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.fpj06012x
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 8544758
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8544758
• Web Site: https://search.jamas.or.jp/link/ui/2007174049
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可