Application of laser-assisted zona drilling to <italic>in vitro</italic> fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse

• ANZAI Masayuki

  Institute of Advanced Technology, Kinki University

• NISHIWAKI Megumi

  Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

• YANAGI Miho

  ARK Resource Co., Ltd.

• NAKASHIMA Tatsuyuki

  ARK Resource Co., Ltd.

• KANEKO Takehito

  Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University

• TAGUCHI Yoshitomo

  Institute of Advanced Technology, Kinki University

• TOKORO Mikiko

  Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

• SHIN Seung-Wook

  Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

• MITANI Tasuku

  Institute of Advanced Technology, Kinki University

• KATO Hiromi

  Institute of Advanced Technology, Kinki University

• MATSUMOTO Kazuya

  Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

• NAKAGATA Naomi

  Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University

• IRITANI Akira

  Institute of Advanced Technology, Kinki University Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University

書誌事項

タイトル別名

• Application of Laser-Assisted Zona Drilling to In Vitro Fertilization of Cryopreserved Mouse Oocytes with Spermatozoa from a Subfertile Transgenic Mouse

抄録

Development of assisted reproductive technologies is necessary to obtain fertilized oocytes in a subfertile transgenic mouse strain. Here, we showed the application of laser-assisted drilling of the zona pellucida to in vitro fertilization of cryopreserved mouse oocytes with sperm from subfertile transgenic mice (C57BL/6N-Tg(UCP/FAD2)U8 strain). After cryopreservation by vitrification, the recovery and survival rates of the zona-drilled mouse oocytes were 97% (97/100) and 94% (91/97), respectively. In vitro fertilization of the cryopreserved zona-drilled mouse oocytes with sperm from the subfertile transgenic mice was greatly facilitated (60%, 55/91) compared to that of the cryopreserved zona-intact mouse oocytes (11%, 81/768). In vitro fertilized embryos that developed to the 2-cell stage were again cryopreserved by vitrification, and after warming they were transferred into recipient females. Subsequently, six viable offspring were delivered, and all were confirmed to be transgenic mice. These results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 52 (5), 601-606, 2006

  公益社団法人 日本繁殖生物学会

キーワード

• <i>In vitro</i> fertilization
• Subfertile
• Transgenic mouse
• Vitrification

詳細情報

• CRID: 1390001206337039488
• NII論文ID: 10020351130
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.18040
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 8513999
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8513999
• Web Site: http://www.jstage.jst.go.jp/article/jrd/52/5/52_5_601/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007173475
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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