Possible Roles of Intracellular Cyclic AMP, Protein Kinase C and Calcium Ion in the Apoptotic Signaling Pathway in Bovine Luteal Cells

• TATSUKAWA Yuko

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

• BOWOLAKSONO Anom

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

• NISHIMURA Ryo

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

• KOMIYAMA Junichi

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

• ACOSTA Tomas J.

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

• OKUDA Kiyoshi

  Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

抄録

Structural luteolysis occurs by apoptosis of luteal cells. The present study examined the effects of activators of well-characterized second messengers on Fas and caspase-3 mRNA expression and on P4 production in luteal cells in order to trace the pro- and anti-apoptotic factors in the bovine corpus luteum (CL). Cultured bovine mid luteal cells were treated for 24 h with a cyclic AMP analogue (8-bromo cyclic AMP; 8br-cAMP; 2.5 mM), a protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate; PMA; 10 μM), or calcium ionophore (A23187; 10 μM). Fas and caspase-3 mRNA expression was inhibited by 8br-cAMP and PMA but was increased by A23187 (P<0.05). In addition, P4 production by bovine luteal cells was stimulated by 8br-cAMP and PMA, whereas it was inhibited by A23187, compared with untreated controls (P<0.05). The overall results suggest that cAMP and PKC suppress apoptosis in bovine luteal cells through inhibition of Fas and caspase-3 mRNA expression and through stimulation of P4 production. Therefore, substances that activate cAMP or PKC may act as survival factors in the bovine CL. Furthermore, substances that mobilize Ca²⁺ may act as apoptotic factors by stimulating Fas and caspase-3 expression in the bovine luteal cells.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 52 (4), 517-522, 2006

  公益社団法人 日本繁殖生物学会

キーワード

• Corpus luteum
• Apoptosis
• Anti-apoptotic factor
• Second messenger
• Cow

詳細情報

• CRID: 1390282681313146240
• NII論文ID: 10020351661
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.18024
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 8019826
• Web Site: http://id.ndl.go.jp/bib/8019826
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8019826
• Web Site: http://www.jstage.jst.go.jp/article/jrd/52/4/52_4_517/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007174269
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可