Karyotype and nuclear DNA content of Psychotria ipecacuanha: a medicinal species

• Rossi Ana Aparecida Bandini

  Departamento de Bioquímica e Biologia Molecular. BIOAGRO. Universidade Federal de Viçosa Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa Departamento de Ciências Biológicas. Universidade do Estado de Mato Grosso-UNEMAT

• Clarindo Wellington Ronildo

  Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa

• Carvalho Carlos Roberto

  Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa

• de Oliveira Luiz Orlando

  Departamento de Bioquímica e Biologia Molecular. BIOAGRO. Universidade Federal de Viçosa

抄録

Psychotria ipecacuanha, ipecac, is a perennial, medicinal herb that grows in clusters in the understory of humid and shady areas of the Brazilian Atlantic and Amazon forests. Cytogenetic studies performed in this species have reported 2n=22 chromosomes, with limited karyotypic information. The present study was conducted in order to apply cytogenetic and flow cytometric tools in samples of ten ipecac populations, five from the Atlantic and five from the Amazon forest, with the purpose of characterizing their karyotype and measuring their 2C DNA content. The flow cytometry analyses evidenced two groups with distinct DNA amounts: MOZ population with 2C=1.24 pg and GUA, TVI, TLM, VRB, RAP, MAN, RVE, BBU and PRA populations with 2C=2.05 pg. Metaphasic chromosomes obtained from root apical meristems of GUA, TLM and TVI showed a karyotype consisting of 11 chromosome pairs, 4 metacentric (4, 5, 8 and 9) and 7 submetacentric (1, 2, 3, 6, 7, 10 and 11), with lengths varying from 3.97 to 2.53 μm. The secondary constriction was identified in the long arm of chromosome 6. Cytogenetic characterization also reveled that each chromosome pair 2–3; 4–5; 6–7; 8–9 and 10–11 is cytogenetically similar, with regard to total length, short and long arm size, and chromosomic class, suggesting that this species might be a tetraploid plant.

収録刊行物

• ＣＹＴＯＬＯＧＩＡ

  ＣＹＴＯＬＯＧＩＡ 73 (1), 53-60, 2008

  公益財団法人 日本メンデル協会

キーワード

• 2C DNA content
• Chromosome
• Cytogenetics
• Flow cytometry
• Ipecac
• <i>Psychotria ipecacuanha</i>

詳細情報

• CRID: 1390001204110800896
• NII論文ID: 10020837300
• NII書誌ID: AA00622764
• DOI: 10.1508/cytologia.73.53
• ISSN: 13487019
• ISSN: 00114545
• Web Site: http://www.jstage.jst.go.jp/article/cytologia/73/1/73_1_53/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可