Tryptophan Neutral Radical Brings along Photochemical Crystallization

• Kenji Furuta

  Department of Chemistry, Gunma University

• Yoshinori Tanizawa

  Department of Chemistry, Gunma University

• Hiroaki Horiuchi

  Department of Chemistry, Gunma University

• Hiroshi Hiratsuka

  Department of Chemistry, Gunma University

• Tetsuo Okutsu

  Department of Chemistry, Gunma University

Abstract

The mechanism of the photochemically induced nucleation of lysozyme was investigated. Radical cations and neutral radicals of tryptophan residue of lysozyme were observed by transient absorption measurements. The radical cation was found to be in equilibrium with the neutral radical, and the pKa of the radical cation in UV–vis wide range buffer solution was estimated to be ca. 3.2. The dimer formation was confirmed by SDS-PAGE for the solutions with pH value higher than this pKa value. The photochemically induced nucleation was also observed for these solutions. The number of crystals increased with increase of the dimer.

Journal

• Chemistry Letters

  Chemistry Letters 37 (4), 458-459, 2008-03-25

  Oxford University Press (OUP)

Keywords

• General Chemistry

Details

• CRID: 1360565169064567296
• NII Article ID: 10021079817
• NII Book ID: AA00603318
• DOI: 10.1246/cl.2008.458
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/37/4/458/55596876/cl.2008.458.pdf
• Data Source:
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  • CiNii Articles