Cell-free Protein Synthesis Conducted by Template DNA with Repetitive Sequence

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  • Takahiko Nojima
    Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo
  • Hiroshi Kimura
    Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo
  • Teruo Fujii
    Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo

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<jats:title>Abstract</jats:title> <jats:p>DNA with repetitive sequence of green fluorescent protein (GFP) was prepared by rolling circle amplification with φ29 DNA polymerase from a bacterial expression vector carrying a GFP gene. The prepared DNA was subjected to a cell-free coupled transcription–translation system and green fluorescence was observed, which shows that the template with repetitive sequence properly functioned in the reaction. The efficiency of the reaction conducted by the template with repetitive sequence was higher than those by linear template coding single gene or by circular plasmid.</jats:p>

収録刊行物

  • Chemistry Letters

    Chemistry Letters 37 (6), 648-649, 2008-05-17

    Oxford University Press (OUP)

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