超高エネルギーX線を利用したタンパク質結晶の位相決定 [in Japanese] Phase Determination of Protein Crystals Using Ultra-High Energy X-rays [in Japanese]
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Structural information of biological macromolecules at an atomic level is indispensable for the current biological studies. In order to investigate the practicality of ultra-high energy X-rays (-35 keV) in protein crystallography, Xe-MAD and I-MAD experiments were performed at the synchrotron facility, SPring-8. The electron density maps for both cases were sufficient enough for auto-tracing of protein structures. Therefore, the use of ultra-high energy X-rays can provide significant development to the high-throughput structure determination of macromolecules. Other studies using ultra-high energy X-rays in protein crystallography are also reviewed in this report.
X-RAYS 50(3), 206-211, 2008-06-30
The Crystallographic Society of Japan