書誌事項
- タイトル別名
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- Comparative Characterization of Ribosomal Proteins of Lactic Acid Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
- マトリックス シエン レーザー ダツリ イオンカ シツリョウ ブンセキホウ ニ ヨル ニュウサンキン ゲノム カイドクカブ ノ リボソーム タンパクシツ ノ ヒカク カイセキ
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抄録
Ribosomal subunit proteins of eight genome-sequenced lactic acid bacteria (LAB) were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to provide a guideline for selecting reliable biomarkers used for the rapid identification of LAB. Subunit proteins with molecular weight over ca. 20,000 were typically difficult to detect. This phenomenon appeared to be due to the mass discrimination effect in MALDI-MS. Conserved post-translational modifications excluding N-terminal methionine loss were investigated by comparing the mass differences from sequence masses calculated from the amino acid sequences of the proteins with the post-translational modifications. Various errors in the amino acid sequences of the subunit proteins registered in protein databases could be detected by comparing amino acid sequences, and corrected by inspecting gene sequences. By eliminating the subunit proteins with higher molecular weight and post-translational modifications as well as other difficult to detect subunit proteins, 27 subunit proteins were finally selected as the reliable biomarkers.
収録刊行物
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- 質量分析
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質量分析 56 (1), 1-11, 2008
一般社団法人 日本質量分析学会
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詳細情報 詳細情報について
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- CRID
- 1390282681473709440
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- NII論文ID
- 10021086448
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- NII書誌ID
- AN0010555X
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL書誌ID
- 9371399
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可