Evaluation of in <i>Vitro Embryotoxicity</i> of Dental Monomers by Differentiation of Embryonic Stem Cells into Contracting Cardiac Myocytes

• Imai Koichi

  Department of Biomaterials, Osaka Dental University

• Hayakawa Toru

  Department of Dental Biomaterials, Nihon University. School of Dentistry at Matsudo

• Nakamura Masaaki

  Department of Biomaterials, Osaka Dental University

Abstract

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3 cells) were examined using the embryonic stem cell test (EST). Three study parameters, the rate of differentiation into contracting cardiomyocytes, the 50% cell viability of ES-D3 and BALB/c 3T3 cells, and the clone A31 (3T3 cells), were used to determine the embryotoxicity. Of the eighteen dental monomers tested, Bis-GMA (6F) and MTYA were classified as weakly embryotoxic, and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, 6-HHMA, MEPC, Phosmer M, BPE-1300, BSNa, EDMABA, GAM, GMR, PTSNa, and QTX were evaluated as nonembryotoxic. However, none of the monomers tested were found to be strongly embryotoxic.<br>Our results suggest that further intensive studies on the embryotoxicity of dental biomaterials are needed in addition to examination of conventional biological aspects from the perspective of biological safety.

Journal

• Alternatives to Animal Testing and Experimentation

  Alternatives to Animal Testing and Experimentation 12 (2), 171-178, 2007

  Japanese Society for Alternative to Animal Experiments

Keywords

• dental Monomer
• embryotoxicity
• EST

Details

• CRID: 1390001205233475200
• NII Article ID: 130004556175
• NII Article ID: 10021095037
• NII Book ID: AA11161787
• DOI: 10.11232/aatex.12.171
• ISSN: 21854726
• ISSN: 13440411
• Web Site: https://search.jamas.or.jp/link/ui/2008017005
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed